Oh my goodness, yes. When I lived in Florida, my town was a resort coastal town near an AF base and everyone there mostly had neutral "accents", but go 30 miles inland and the southern drawl took over. Most of NW Florida is just underneath Alabama and very close to the state line. A little further east and North Central Florida is under the Georgia state line, so a southern drawl again, but slightly different. Many of the tourists had deep southern accents. The Emerald Coast of NW Florida is also know as the Redneck Riviera, by the way, because people from Tennessee, the Carolinas, etc. would come vacation there.
Here's a story I have told before:
I was hanging out at a bar with some friends in my hometown of Destin, FL back when I was about 25. Anywhere in FL in beach resort areas, most people don't have much of a Southern accent, if any. My friends and I did not. Anyway, some guy came up to me and asked if he could buy me a drink. He had a very deep drawl. I asked him where he was from, and he said what sounded to me like, "Way across Georgia." I asked, "Yeah but where are you from?" and he said, "Way across." I said, "Yeah, but how far across, and what's the name of the town?" He shouted at me, "THE NAME OF THE TOWN IS WAYCROSS!" My friends and I busted up laughing and he walked away.
